IGF1-LR3 1mg

🔬 Product Code: IGF1-LR3-1MG

🧪 Specification: 1mg Lyophilized Powder

🧬 Purity: ≥99% Verified via HPLC / MS

⚠️ Application: For In-Vitro and Preclinical Laboratory Research Only. Not for human or veterinary use.

Product Overview

IGF1-LR3 (Long Arginine 3 Insulin-like Growth Factor-1) is a synthetic, highly potent analog of naturally occurring human Insulin-like Growth Factor-1 ($\text{IGF-1}$). Structurally, it consists of the complete 70-amino acid $\text{IGF-1}$ sequence, but features two distinct modifications: the substitution of an Arginine for Glutamic Acid at position 3 (hence “R3”), and a 13-amino acid extension peptide added at the N-terminus (making it “Long”).

In cellular biology and physiological research, these deliberate structural engineering changes solve a major baseline hurdle: the high affinity of standard $\text{IGF-1}$ for IGF-Binding Proteins (IGFBPs). While native $\text{IGF-1}$ is quickly bound and deactivated by these proteins in a biological matrix, IGF1-LR3 displays a drastically reduced binding affinity for IGFBPs.

Because it remains free and active rather than bound, IGF1-LR3 features a profoundly extended half-life of approximately 20 to 30 hours (compared to the brief 20-minute half-life of native $\text{IGF-1}$). This permits continuous, uninterrupted activation of the IGF-1 receptor (IGF-1R), triggering sustained downstream intracellular signaling cascades via the PI3K/Akt pathway.

Key Research Applications

• Hyperplasia and Myogenesis Studies: Investigating the proliferation, differentiation, and fusing of satellite muscle cells into new, mature skeletal muscle fibers in cellular assays.

• Intracellular Nutrient Transport: Analyzing the upregulation of glycogen synthesis and amino acid transport across cell membranes to model lean tissue nutrient partitioning.

• Long-Term Receptor Saturation: Evaluating the systemic cellular adaptations, protein synthesis markers, and survival pathways under continuous IGF-1R activation without binding-protein interference.

Uncompromising Quality Control

Every batch of IGF1-LR3 distributed by Onyx Research undergoes independent, third-party analytical testing to guarantee reproducible experimental results.

• High-Performance Liquid Chromatography (HPLC): Confirms an exact purity profile exceeding 99%.

• Mass Spectrometry (MS): Verifies correct sequence identity and precise molecular mass ($9,111\text{ Da}$).

• Lyophilization Technology: Vacuum-sealed to ensure optimal structural stability during transport and storage.

Handling & Storage Guidelines

• Form: Lyophilized (freeze-dried) white crystalline powder.

• Reconstitution: For laboratory testing, reconstitute using sterile bacteriostatic water. For prolonged shelf life or highly sensitive cell culture models, a weak acid solution (such as 10mM or 0.1M acetic acid) can be used before adding a buffer. Avoid vigorous agitation to prevent structural shearing of the large protein molecule.

• Storage (Lyophilized): Stable at room temperature for up to 4 weeks during transit. Store long-term at $-20^\circ\text{C}$ to preserve peptide integrity for up to 24 months.

• Storage (Reconstituted): Once in solution, store under refrigeration at $2^\circ\text{C}$ to $8^\circ\text{C}$ and utilize within 10 to 14 days for optimal experimental consistency.

Laboratory Safety Disclaimer: This compound is supplied exclusively to qualified professionals, academic institutions, and commercial laboratories engaged in scientific innovation. Onyx Research explicitly prohibits the administration of this material to humans or animals. All handling must conform to strict laboratory safety standards, including the use of proper personal protective equipment (PPE).