NAD+ 500MG

🔬 Product Code: NAD-500MG

🧪 Specification: 500mg Lyophilized Powder

🧬 Purity: ≥99% Verified via HPLC / MS

⚠️ Application: For In-Vitro and Preclinical Laboratory Research Only. Not for human or veterinary use.

Product Overview

NAD+ (Nicotinamide Adenine Dinucleotide) is an essential coenzyme found in every living cell, functioning as a primary catalyst for cellular energy production and metabolic homeostasis. This formulation provides 500mg of highly purified, stable NAD+ in its oxidized form, ready for exact quantitative reconstitution in scientific assays.

In cellular biology, biochemistry, and longevity research, NAD+ serves as the fundamental electron carrier in redox reactions, shifting between NAD+ and NADH to drive the mitochondrial electron transport chain for Adenosine Triphosphate (ATP) synthesis. Beyond its role in energy production, NAD+ acts as an obligatory substrate for key regulatory enzymes:

• Sirtuins (SIRT1-SIRT7): Deacetylase enzymes that depend on NAD+ to regulate epigenetic gene expression, DNA repair, mitochondrial biogenesis, and cellular survival pathways.

• PARPs (Poly ADP-Ribose Polymerases): Enzymes that consume NAD+ to detect and repair damaged DNA strands, maintaining genomic stability.

• CD38/CD157: Ectoenzymes that utilize NAD+ to generate secondary messengers for intracellular calcium signaling.

Because systemic NAD+ levels decline drastically with cellular aging and metabolic stress, isolating this coenzyme in laboratory models is paramount to studying the reversal of mitochondrial decay, cellular senescence, and age-related physiological decline.

Key Research Applications

• Mitochondrial Bioenergetics: Investigating the correlation between intracellular NAD+/NADH ratios and mitochondrial respiration efficiency or ATP output.

• Epigenetic Modification and Longevity: Mapping sirtuin-mediated pathways to evaluate chromatin remodeling, histone deacetylation, and cellular lifespan extension in animal models.

• DNA Repair Mechanics: Evaluating PARP activation and DNA strand break repair rates under conditions of induced oxidative or chemical stress.

Uncompromising Quality Control

Every batch of NAD+ distributed by Onyx Research undergoes independent, third-party analytical testing to guarantee absolute data integrity.

• High-Performance Liquid Chromatography (HPLC): Confirms a baseline purity profile exceeding 99%.

• Mass Spectrometry (MS): Verifies correct molecular mass and identity ($663.4\text{ Da}$), ensuring complete structural authenticity.

• Lyophilization Technology: Vacuum-sealed in clinical-grade glass vials to preserve maximum molecular stability and prevent premature degradation.

Handling & Storage Guidelines

• Form: Lyophilized (freeze-dried) white to off-white crystalline powder.

• Reconstitution: Reconstitute using sterile bacteriostatic water or sterile physiological saline depending on your assay protocol. Guide the fluid gently down the inner glass wall of the vial and swirl slowly until fully dissolved.

• Storage (Lyophilized): Highly hygroscopic. Store long-term at $-20^\circ\text{C}$ to avoid moisture absorption and hydrolytic degradation. Kept frozen, the compound remains stable for up to 24 months.

• Storage (Reconstituted): Once in solution, NAD+ is susceptible to rapid hydrolysis. Store under refrigeration at $2^\circ\text{C}$ to $8^\circ\text{C}$ and utilize within 24 to 48 hours for absolute experimental precision, or aliquot and freeze at $-80^\circ\text{C}$ for extended solution stability.

Laboratory Safety Disclaimer: This compound is supplied exclusively to qualified professionals, academic institutions, and commercial laboratories engaged in scientific innovation. Onyx Research explicitly prohibits the administration of this material to humans or animals. All handling must conform to strict laboratory safety standards, including the use of proper personal protective equipment (PPE).